Effect of Changes in Substrate Concentration on the Reaction Rate

Effect of changes in substratum stringency on the reply tempo of an enzyme IB biology Internal judging 3/23/12 inquiry Question Effect of changes in subst outrank niggardness sum total on the reply rate of an enzyme Introduction In this look into, the substrate is atomic number 1 bl apiece. The purpose of this probe is to regain out the relationship between the substrate niggardliness and the rate of chemical reply. Substrates ar molecules that ar acted upon by enzymes. For instance, amylase, an enzyme found in saliva, helps get word down complex starch molecules (substrates) into littler sugar molecules (products).In other biochemical re swear outs, substrates supplicate assistance of specific enzymes to form naked products. When the amount of enzyme stays regular, the substrate c erstntration will determine the rate of reception. However, when the rate of substrate molecules exceeds the available number of enzyme, the rate of response will no great incr ease, but stay constant. If at that place is a constant amount of enzyme, as the concentration of a substrate increased, the rate of reaction will increase as well. This is because of molecular collisions.If you do more reactant molecules, there are more to collide. Aim The effect of enthalpy atomic number 1 hydrogen peroxide on the enzyme activity of catalase assumption When the amount of enzyme stays constant, the substrate concentration will determine the rate of reaction CONTROLLED VARIABLES Units Possible effect(s) on results Amount of enzyme 2. 8g an additional drop of enzyme kindle alter the rate of reaction Size and type of raise vacuum pipages 30ml The size and type of streamlet organ pipes were constant, because they can alter the pressure Units Range self-sufficient VARIABLE Hydrogen Peroxide (Substrate) assiduousness ml 5,10,15,20,25,30 DEPENDENDENT VARIABLE Rate of reaction Seconds 80 secs VARIABLES METHOD FOR CONTROLLING VARIABLES CONTROLLED VA RIABLES rule for control 1. Amount of enzyme All liver-colored use were at a constant weight of 2. 8g 2. Size of study electron thermionic valve All test furnishs were 30ml METHOD FOR collect DATA 1. Prepare a tube rack and place 6 30ml tubes in them. 2. Weigh liver at a constant 2. 8g. 3. Place the 6 pieces of liver into the test tubes. 4.Obtain 3% hydrogen peroxide and a graduate cylinder. 5. Pour 5ml into test tube 1, 10ml into test tube 2, 15ml into test tube, 20ml into test tube 4, 25 ml into test tube 5, 30ml into test tube 6 (but not at once one after the another) . 6. Once hydrogen is in the test tube opening the stop watch to see how long it will take to react. 7. cite the action in no. 5 & international ampere 6, six sequences for each tube. 8. find what happens to the liver while reacting to the hydrogen peroxide. 9. extend up the station and pour liver into a waste beaker. 0. Clean each of the test tubes out and effectuate the materials away. The materia ls used in this experiment are I. 50-ml graduated cylinder II. Fresh liver III. 6 test tubes (30 ml) IV. 3% Hydrogen peroxide V. Disposable Pipettes VI. Stopwatch VII. Digital carapace VIII. 50ml beaker IX. Test tube rack X. tensile knife XI. Scissors QUALITATIVE DATA. The reaction started as soon as Catalase stirred the scrape up of hydrogen peroxide. More intemperate hydrogen peroxide produced more type O bubbles and the reaction rate was faster.As more substrate was added the reaction was faster. Once the 5ml of hydrogen peroxide was put into the test tube with the liver, the reaction rate was slow. As the amount of hydrogen peroxide increased the reaction became faster. When move the 15ml of peroxide into the test tube 3 during the freshman trial the reaction bubbles spilled into tube 4 affecting the result slightly, because it do it to start reacting before the 20ml of peroxide was put into test tube 4 . In test tube 6 during the first trial the liver was lifted from th e surface about 2cm.The color for test tubes 1-5 during either the six trials was light brown, but for tube six the color was dark brown. in front SUBSTRATE AFTER SUBSTRATE save dim DATAPROCESSING RAW DATA Amount of Solute concentration (ml) Repeat response time (s)(+/-0. 5s) 5 1 one hundred thirty 2 129 3 130 4 132 5 128 6 123 10 1 100 2 cx 3 92 4 98 5 95 6 hundred and one 15 1 87 2 87 3 84 4 88 5 82 6 84 20 1 63 2 70 3 78 4 71 5 74 6 75 25 1 59 2 58 3 60 4 60 5 58 6 59 0 1 39 2 42 3 37 4 41 5 40 6 38 Amount of Solute concentration (ml) Repeat Reaction time (s)(+/-0. 5s) Mean (s)(+/-0. 5s) 5 1 130 128. 6 2 129 3 130 4 132 5 128 6 123 10 1 100 99. 3 2 110 3 92 4 98 5 95 6 101 15 1 87 85. 3 2 87 3 84 4 88 5 82 6 84 20 1 63 71. 8 2 70 3 78 4 71 5 74 6 75 25 1 59 59. 0 2 58 3 60 4 60 5 58 6 59 30 1 39 39. 5 2 42 3 37 4 41 5 40 6 38 *Sample computation of mean sum of reaction time for tube/ o f trials 39+42+37+41+40+38=237 237/6= 39. 5 PRESENTING PROCESSED DATA lowest My hypothesis was supported based on my data. The data suggests that as the hydrogen peroxide concentration increases the rate of reaction increased. It took slight time for it to react according to betoken 1. The general trend that was in this experiment was that the numbers for each amount of hydrogen were in the same range e. g. 15ml (87 87 84 88 82 84).My prophecy was correct the more substrate was added the little time it used to react thusly a faster reaction rate. on that point were no anomalous results. The data in this experiment suggests that the change in amount of substrate creates a faster reaction rate. EVALUATING PROCEDURES Even though the experiment and the final result of the experiment support my hypothesis there are some weakness in this experiment that would rent enabled a meliorate outcome. The weaknesses that were present in the in the rule of chosen for this investigation wa s the size of liver.The expire weakness the arrangement in the go interpreted. IMPROVING THE INVESTIGATION To improve the results of this investigation is the size of liver should have been smaller, so that more reaction would have taken place and the color of the liver would have changed more for all of the tubes. Another good would be in the arrangement of go taken. To avoid the spillover of the reaction bubbles into test tube 4, the amount of hydrogen peroxide should have been in the test tubes first wherefore the liver should have been dropped in after.